Host/Virus Expression

Sharing Knowledge: Working with Host/Virus Systems

Why choose a Host/Virus system for Expression?

The use of viruses to produce important therapies including gene therapies have become an important modality in the treatment of many disorders. Selecting the right combination will depend mainly on safety, toxicity, and the ability of the host/virus combination to effectively produce the target product.

Typical Virus Replication platforms hosts are:
  • HK-293-  these cells typically serve as excellent plasmids to make AAV virus. Also used in the production of lentiviruses.  Typically are compatible with suspension processes.
  • Per.C6- human cell line, characterized for the propagation on Influenza A and B.
  • Sf9/Sf21- well established systems with regulatory traction.
  • CHO- well proven, characterized, and established host.
  • EB66- typically used for antibody production.
  • Vero- these systems already have regulatory traction with an approved product. Manufacturing challenges as typically are not compatible with suspension processes.
  • MDCK- typically have good virus growth advantage, adapt well to serum free media.
Typical Viral Vectors include:
  • Adenovirus
  • Adeno-associated virus (AAV)
  • Lentivirus
  • Poxvirus
  • Baculovirus

What are the challenges of working with Host/Virus systems?

Developing a long-term manufacturing ready process can be a challenge when working with Host/virus systems. Host cells have different growth properties, including adherent versus suspension cultures. This may require the evaluation of alternate platforms such as Hyperstacks™, Cell Factory™ systems and/or microcarriers. Some host cells, such as HEK, may have media requirements that can be challenging.

Purification strategies for long term success are important to consider while developing a process. Ultracentrifugation for virus purification is typically not a scalable solution long term. Evaluation of purification by chromatography should be considered early during process development.

How do we help?

At FUJIFILM Diosynth Biotechnologies we have the capability to support our customers in the production of their viral products from early preclinical stage all the way to commercial production. We have an established group of virologists that bring many years of hands on experience with a wide range of systems.